Software:Article Title: Senescence-associated lysosomal dysfunction impairs cystine deprivation-induced lipid peroxidation and ferroptosis
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Data analysis Akiko Takahashi Jun 27, 2025 Confocal microscopic data: ECLIPSE Ti2-E, Nikon AX Quantitative real-time PCR data: StepOnePlus PCR system (Applied Biosystems) Immunoblotting data: FUSION imaging system (Vilber-Lourmat) Histological data: Fluorescence microscope (Olymplus, BX51) Fluorescence imaging analysis: Operetta CLS (PerkinElmer) Flow cytometry: Spectral analyzer SA3800 (SONY) scRNA-Seq library preparation: BD Rhapsody System (BD Biosciences) Single-cell RNA sequencing: NEB Next UltraIIFS Library prep kit (New England Biolabs) and Illumina Novaseq 6000 sequencer Fluorescence and absorbance measurement: Nivo (PerkinElmer) Liquid chromatography-tandem mass spectrometry: Triple-quadrupole mass spectrometer (Shimadzu Corporation,LCMS-8060) Analysis of (oxidized) lipids: Orbitrap Exploris mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA) linked with Ultimate 3000 HPLC system (Thermo Fisher Scientific) Immunofluorescence staining analysis: NIH Image J software (version 1.57) Statistical analysis and visualization: R version 4.4.1 (2024-06-14) Statistical analysis: GraphPad Prism 10 (version 10.4.2) Visualization: Illustrator (version 29.0.1) Assessment of oxidized lipids: Qual Browser (Thermo Fisher Scientific) Metabolome analysis: Metaboanalyst 6.0 2 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
Real-time Polymerase Chain Reaction:Article Title: Senescence-associated lysosomal dysfunction impairs cystine deprivation-induced lipid peroxidation and ferroptosis
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Data analysis Akiko Takahashi Jun 27, 2025 Confocal microscopic data: ECLIPSE Ti2-E, Nikon AX Quantitative real-time PCR data: StepOnePlus PCR system (Applied Biosystems) Immunoblotting data: FUSION imaging system (Vilber-Lourmat) Histological data: Fluorescence microscope (Olymplus, BX51) Fluorescence imaging analysis: Operetta CLS (PerkinElmer) Flow cytometry: Spectral analyzer SA3800 (SONY) scRNA-Seq library preparation: BD Rhapsody System (BD Biosciences) Single-cell RNA sequencing: NEB Next UltraIIFS Library prep kit (New England Biolabs) and Illumina Novaseq 6000 sequencer Fluorescence and absorbance measurement: Nivo (PerkinElmer) Liquid chromatography-tandem mass spectrometry: Triple-quadrupole mass spectrometer (Shimadzu Corporation,LCMS-8060) Analysis of (oxidized) lipids: Orbitrap Exploris mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA) linked with Ultimate 3000 HPLC system (Thermo Fisher Scientific) Immunofluorescence staining analysis: NIH Image J software (version 1.57) Statistical analysis and visualization: R version 4.4.1 (2024-06-14) Statistical analysis: GraphPad Prism 10 (version 10.4.2) Visualization: Illustrator (version 29.0.1) Assessment of oxidized lipids: Qual Browser (Thermo Fisher Scientific) Metabolome analysis: Metaboanalyst 6.0 2 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
Polymerase Chain Reaction:Article Title: Senescence-associated lysosomal dysfunction impairs cystine deprivation-induced lipid peroxidation and ferroptosis
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Data analysis Akiko Takahashi Jun 27, 2025 Confocal microscopic data: ECLIPSE Ti2-E, Nikon AX Quantitative real-time PCR data: StepOnePlus PCR system (Applied Biosystems) Immunoblotting data: FUSION imaging system (Vilber-Lourmat) Histological data: Fluorescence microscope (Olymplus, BX51) Fluorescence imaging analysis: Operetta CLS (PerkinElmer) Flow cytometry: Spectral analyzer SA3800 (SONY) scRNA-Seq library preparation: BD Rhapsody System (BD Biosciences) Single-cell RNA sequencing: NEB Next UltraIIFS Library prep kit (New England Biolabs) and Illumina Novaseq 6000 sequencer Fluorescence and absorbance measurement: Nivo (PerkinElmer) Liquid chromatography-tandem mass spectrometry: Triple-quadrupole mass spectrometer (Shimadzu Corporation,LCMS-8060) Analysis of (oxidized) lipids: Orbitrap Exploris mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA) linked with Ultimate 3000 HPLC system (Thermo Fisher Scientific) Immunofluorescence staining analysis: NIH Image J software (version 1.57) Statistical analysis and visualization: R version 4.4.1 (2024-06-14) Statistical analysis: GraphPad Prism 10 (version 10.4.2) Visualization: Illustrator (version 29.0.1) Assessment of oxidized lipids: Qual Browser (Thermo Fisher Scientific) Metabolome analysis: Metaboanalyst 6.0 2 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
Western Blot:Article Title: Senescence-associated lysosomal dysfunction impairs cystine deprivation-induced lipid peroxidation and ferroptosis
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Data analysis Akiko Takahashi Jun 27, 2025 Confocal microscopic data: ECLIPSE Ti2-E, Nikon AX Quantitative real-time PCR data: StepOnePlus PCR system (Applied Biosystems) Immunoblotting data: FUSION imaging system (Vilber-Lourmat) Histological data: Fluorescence microscope (Olymplus, BX51) Fluorescence imaging analysis: Operetta CLS (PerkinElmer) Flow cytometry: Spectral analyzer SA3800 (SONY) scRNA-Seq library preparation: BD Rhapsody System (BD Biosciences) Single-cell RNA sequencing: NEB Next UltraIIFS Library prep kit (New England Biolabs) and Illumina Novaseq 6000 sequencer Fluorescence and absorbance measurement: Nivo (PerkinElmer) Liquid chromatography-tandem mass spectrometry: Triple-quadrupole mass spectrometer (Shimadzu Corporation,LCMS-8060) Analysis of (oxidized) lipids: Orbitrap Exploris mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA) linked with Ultimate 3000 HPLC system (Thermo Fisher Scientific) Immunofluorescence staining analysis: NIH Image J software (version 1.57) Statistical analysis and visualization: R version 4.4.1 (2024-06-14) Statistical analysis: GraphPad Prism 10 (version 10.4.2) Visualization: Illustrator (version 29.0.1) Assessment of oxidized lipids: Qual Browser (Thermo Fisher Scientific) Metabolome analysis: Metaboanalyst 6.0 2 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
Imaging:Article Title: Senescence-associated lysosomal dysfunction impairs cystine deprivation-induced lipid peroxidation and ferroptosis
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Data analysis Akiko Takahashi Jun 27, 2025 Confocal microscopic data: ECLIPSE Ti2-E, Nikon AX Quantitative real-time PCR data: StepOnePlus PCR system (Applied Biosystems) Immunoblotting data: FUSION imaging system (Vilber-Lourmat) Histological data: Fluorescence microscope (Olymplus, BX51) Fluorescence imaging analysis: Operetta CLS (PerkinElmer) Flow cytometry: Spectral analyzer SA3800 (SONY) scRNA-Seq library preparation: BD Rhapsody System (BD Biosciences) Single-cell RNA sequencing: NEB Next UltraIIFS Library prep kit (New England Biolabs) and Illumina Novaseq 6000 sequencer Fluorescence and absorbance measurement: Nivo (PerkinElmer) Liquid chromatography-tandem mass spectrometry: Triple-quadrupole mass spectrometer (Shimadzu Corporation,LCMS-8060) Analysis of (oxidized) lipids: Orbitrap Exploris mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA) linked with Ultimate 3000 HPLC system (Thermo Fisher Scientific) Immunofluorescence staining analysis: NIH Image J software (version 1.57) Statistical analysis and visualization: R version 4.4.1 (2024-06-14) Statistical analysis: GraphPad Prism 10 (version 10.4.2) Visualization: Illustrator (version 29.0.1) Assessment of oxidized lipids: Qual Browser (Thermo Fisher Scientific) Metabolome analysis: Metaboanalyst 6.0 2 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
Fluorescence:Article Title: Senescence-associated lysosomal dysfunction impairs cystine deprivation-induced lipid peroxidation and ferroptosis
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Data analysis Akiko Takahashi Jun 27, 2025 Confocal microscopic data: ECLIPSE Ti2-E, Nikon AX Quantitative real-time PCR data: StepOnePlus PCR system (Applied Biosystems) Immunoblotting data: FUSION imaging system (Vilber-Lourmat) Histological data: Fluorescence microscope (Olymplus, BX51) Fluorescence imaging analysis: Operetta CLS (PerkinElmer) Flow cytometry: Spectral analyzer SA3800 (SONY) scRNA-Seq library preparation: BD Rhapsody System (BD Biosciences) Single-cell RNA sequencing: NEB Next UltraIIFS Library prep kit (New England Biolabs) and Illumina Novaseq 6000 sequencer Fluorescence and absorbance measurement: Nivo (PerkinElmer) Liquid chromatography-tandem mass spectrometry: Triple-quadrupole mass spectrometer (Shimadzu Corporation,LCMS-8060) Analysis of (oxidized) lipids: Orbitrap Exploris mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA) linked with Ultimate 3000 HPLC system (Thermo Fisher Scientific) Immunofluorescence staining analysis: NIH Image J software (version 1.57) Statistical analysis and visualization: R version 4.4.1 (2024-06-14) Statistical analysis: GraphPad Prism 10 (version 10.4.2) Visualization: Illustrator (version 29.0.1) Assessment of oxidized lipids: Qual Browser (Thermo Fisher Scientific) Metabolome analysis: Metaboanalyst 6.0 2 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
Microscopy:Article Title: Senescence-associated lysosomal dysfunction impairs cystine deprivation-induced lipid peroxidation and ferroptosis
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Data analysis Akiko Takahashi Jun 27, 2025 Confocal microscopic data: ECLIPSE Ti2-E, Nikon AX Quantitative real-time PCR data: StepOnePlus PCR system (Applied Biosystems) Immunoblotting data: FUSION imaging system (Vilber-Lourmat) Histological data: Fluorescence microscope (Olymplus, BX51) Fluorescence imaging analysis: Operetta CLS (PerkinElmer) Flow cytometry: Spectral analyzer SA3800 (SONY) scRNA-Seq library preparation: BD Rhapsody System (BD Biosciences) Single-cell RNA sequencing: NEB Next UltraIIFS Library prep kit (New England Biolabs) and Illumina Novaseq 6000 sequencer Fluorescence and absorbance measurement: Nivo (PerkinElmer) Liquid chromatography-tandem mass spectrometry: Triple-quadrupole mass spectrometer (Shimadzu Corporation,LCMS-8060) Analysis of (oxidized) lipids: Orbitrap Exploris mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA) linked with Ultimate 3000 HPLC system (Thermo Fisher Scientific) Immunofluorescence staining analysis: NIH Image J software (version 1.57) Statistical analysis and visualization: R version 4.4.1 (2024-06-14) Statistical analysis: GraphPad Prism 10 (version 10.4.2) Visualization: Illustrator (version 29.0.1) Assessment of oxidized lipids: Qual Browser (Thermo Fisher Scientific) Metabolome analysis: Metaboanalyst 6.0 2 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
Flow Cytometry:Article Title: Senescence-associated lysosomal dysfunction impairs cystine deprivation-induced lipid peroxidation and ferroptosis
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Data analysis Akiko Takahashi Jun 27, 2025 Confocal microscopic data: ECLIPSE Ti2-E, Nikon AX Quantitative real-time PCR data: StepOnePlus PCR system (Applied Biosystems) Immunoblotting data: FUSION imaging system (Vilber-Lourmat) Histological data: Fluorescence microscope (Olymplus, BX51) Fluorescence imaging analysis: Operetta CLS (PerkinElmer) Flow cytometry: Spectral analyzer SA3800 (SONY) scRNA-Seq library preparation: BD Rhapsody System (BD Biosciences) Single-cell RNA sequencing: NEB Next UltraIIFS Library prep kit (New England Biolabs) and Illumina Novaseq 6000 sequencer Fluorescence and absorbance measurement: Nivo (PerkinElmer) Liquid chromatography-tandem mass spectrometry: Triple-quadrupole mass spectrometer (Shimadzu Corporation,LCMS-8060) Analysis of (oxidized) lipids: Orbitrap Exploris mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA) linked with Ultimate 3000 HPLC system (Thermo Fisher Scientific) Immunofluorescence staining analysis: NIH Image J software (version 1.57) Statistical analysis and visualization: R version 4.4.1 (2024-06-14) Statistical analysis: GraphPad Prism 10 (version 10.4.2) Visualization: Illustrator (version 29.0.1) Assessment of oxidized lipids: Qual Browser (Thermo Fisher Scientific) Metabolome analysis: Metaboanalyst 6.0 2 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
Single Cell:Article Title: Senescence-associated lysosomal dysfunction impairs cystine deprivation-induced lipid peroxidation and ferroptosis
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Data analysis Akiko Takahashi Jun 27, 2025 Confocal microscopic data: ECLIPSE Ti2-E, Nikon AX Quantitative real-time PCR data: StepOnePlus PCR system (Applied Biosystems) Immunoblotting data: FUSION imaging system (Vilber-Lourmat) Histological data: Fluorescence microscope (Olymplus, BX51) Fluorescence imaging analysis: Operetta CLS (PerkinElmer) Flow cytometry: Spectral analyzer SA3800 (SONY) scRNA-Seq library preparation: BD Rhapsody System (BD Biosciences) Single-cell RNA sequencing: NEB Next UltraIIFS Library prep kit (New England Biolabs) and Illumina Novaseq 6000 sequencer Fluorescence and absorbance measurement: Nivo (PerkinElmer) Liquid chromatography-tandem mass spectrometry: Triple-quadrupole mass spectrometer (Shimadzu Corporation,LCMS-8060) Analysis of (oxidized) lipids: Orbitrap Exploris mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA) linked with Ultimate 3000 HPLC system (Thermo Fisher Scientific) Immunofluorescence staining analysis: NIH Image J software (version 1.57) Statistical analysis and visualization: R version 4.4.1 (2024-06-14) Statistical analysis: GraphPad Prism 10 (version 10.4.2) Visualization: Illustrator (version 29.0.1) Assessment of oxidized lipids: Qual Browser (Thermo Fisher Scientific) Metabolome analysis: Metaboanalyst 6.0 2 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
RNA Sequencing:Article Title: Senescence-associated lysosomal dysfunction impairs cystine deprivation-induced lipid peroxidation and ferroptosis
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Data analysis Akiko Takahashi Jun 27, 2025 Confocal microscopic data: ECLIPSE Ti2-E, Nikon AX Quantitative real-time PCR data: StepOnePlus PCR system (Applied Biosystems) Immunoblotting data: FUSION imaging system (Vilber-Lourmat) Histological data: Fluorescence microscope (Olymplus, BX51) Fluorescence imaging analysis: Operetta CLS (PerkinElmer) Flow cytometry: Spectral analyzer SA3800 (SONY) scRNA-Seq library preparation: BD Rhapsody System (BD Biosciences) Single-cell RNA sequencing: NEB Next UltraIIFS Library prep kit (New England Biolabs) and Illumina Novaseq 6000 sequencer Fluorescence and absorbance measurement: Nivo (PerkinElmer) Liquid chromatography-tandem mass spectrometry: Triple-quadrupole mass spectrometer (Shimadzu Corporation,LCMS-8060) Analysis of (oxidized) lipids: Orbitrap Exploris mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA) linked with Ultimate 3000 HPLC system (Thermo Fisher Scientific) Immunofluorescence staining analysis: NIH Image J software (version 1.57) Statistical analysis and visualization: R version 4.4.1 (2024-06-14) Statistical analysis: GraphPad Prism 10 (version 10.4.2) Visualization: Illustrator (version 29.0.1) Assessment of oxidized lipids: Qual Browser (Thermo Fisher Scientific) Metabolome analysis: Metaboanalyst 6.0 2 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
Chromatography:Article Title: Senescence-associated lysosomal dysfunction impairs cystine deprivation-induced lipid peroxidation and ferroptosis
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Data analysis Akiko Takahashi Jun 27, 2025 Confocal microscopic data: ECLIPSE Ti2-E, Nikon AX Quantitative real-time PCR data: StepOnePlus PCR system (Applied Biosystems) Immunoblotting data: FUSION imaging system (Vilber-Lourmat) Histological data: Fluorescence microscope (Olymplus, BX51) Fluorescence imaging analysis: Operetta CLS (PerkinElmer) Flow cytometry: Spectral analyzer SA3800 (SONY) scRNA-Seq library preparation: BD Rhapsody System (BD Biosciences) Single-cell RNA sequencing: NEB Next UltraIIFS Library prep kit (New England Biolabs) and Illumina Novaseq 6000 sequencer Fluorescence and absorbance measurement: Nivo (PerkinElmer) Liquid chromatography-tandem mass spectrometry: Triple-quadrupole mass spectrometer (Shimadzu Corporation,LCMS-8060) Analysis of (oxidized) lipids: Orbitrap Exploris mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA) linked with Ultimate 3000 HPLC system (Thermo Fisher Scientific) Immunofluorescence staining analysis: NIH Image J software (version 1.57) Statistical analysis and visualization: R version 4.4.1 (2024-06-14) Statistical analysis: GraphPad Prism 10 (version 10.4.2) Visualization: Illustrator (version 29.0.1) Assessment of oxidized lipids: Qual Browser (Thermo Fisher Scientific) Metabolome analysis: Metaboanalyst 6.0 2 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
Mass Spectrometry:Article Title: Senescence-associated lysosomal dysfunction impairs cystine deprivation-induced lipid peroxidation and ferroptosis
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Data analysis Akiko Takahashi Jun 27, 2025 Confocal microscopic data: ECLIPSE Ti2-E, Nikon AX Quantitative real-time PCR data: StepOnePlus PCR system (Applied Biosystems) Immunoblotting data: FUSION imaging system (Vilber-Lourmat) Histological data: Fluorescence microscope (Olymplus, BX51) Fluorescence imaging analysis: Operetta CLS (PerkinElmer) Flow cytometry: Spectral analyzer SA3800 (SONY) scRNA-Seq library preparation: BD Rhapsody System (BD Biosciences) Single-cell RNA sequencing: NEB Next UltraIIFS Library prep kit (New England Biolabs) and Illumina Novaseq 6000 sequencer Fluorescence and absorbance measurement: Nivo (PerkinElmer) Liquid chromatography-tandem mass spectrometry: Triple-quadrupole mass spectrometer (Shimadzu Corporation,LCMS-8060) Analysis of (oxidized) lipids: Orbitrap Exploris mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA) linked with Ultimate 3000 HPLC system (Thermo Fisher Scientific) Immunofluorescence staining analysis: NIH Image J software (version 1.57) Statistical analysis and visualization: R version 4.4.1 (2024-06-14) Statistical analysis: GraphPad Prism 10 (version 10.4.2) Visualization: Illustrator (version 29.0.1) Assessment of oxidized lipids: Qual Browser (Thermo Fisher Scientific) Metabolome analysis: Metaboanalyst 6.0 2 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
Targeted Proteomics:Article Title: Senescence-associated lysosomal dysfunction impairs cystine deprivation-induced lipid peroxidation and ferroptosis
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Data analysis Akiko Takahashi Jun 27, 2025 Confocal microscopic data: ECLIPSE Ti2-E, Nikon AX Quantitative real-time PCR data: StepOnePlus PCR system (Applied Biosystems) Immunoblotting data: FUSION imaging system (Vilber-Lourmat) Histological data: Fluorescence microscope (Olymplus, BX51) Fluorescence imaging analysis: Operetta CLS (PerkinElmer) Flow cytometry: Spectral analyzer SA3800 (SONY) scRNA-Seq library preparation: BD Rhapsody System (BD Biosciences) Single-cell RNA sequencing: NEB Next UltraIIFS Library prep kit (New England Biolabs) and Illumina Novaseq 6000 sequencer Fluorescence and absorbance measurement: Nivo (PerkinElmer) Liquid chromatography-tandem mass spectrometry: Triple-quadrupole mass spectrometer (Shimadzu Corporation,LCMS-8060) Analysis of (oxidized) lipids: Orbitrap Exploris mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA) linked with Ultimate 3000 HPLC system (Thermo Fisher Scientific) Immunofluorescence staining analysis: NIH Image J software (version 1.57) Statistical analysis and visualization: R version 4.4.1 (2024-06-14) Statistical analysis: GraphPad Prism 10 (version 10.4.2) Visualization: Illustrator (version 29.0.1) Assessment of oxidized lipids: Qual Browser (Thermo Fisher Scientific) Metabolome analysis: Metaboanalyst 6.0 2 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
High Performance Liquid Chromatography:Article Title: Senescence-associated lysosomal dysfunction impairs cystine deprivation-induced lipid peroxidation and ferroptosis
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Data analysis Akiko Takahashi Jun 27, 2025 Confocal microscopic data: ECLIPSE Ti2-E, Nikon AX Quantitative real-time PCR data: StepOnePlus PCR system (Applied Biosystems) Immunoblotting data: FUSION imaging system (Vilber-Lourmat) Histological data: Fluorescence microscope (Olymplus, BX51) Fluorescence imaging analysis: Operetta CLS (PerkinElmer) Flow cytometry: Spectral analyzer SA3800 (SONY) scRNA-Seq library preparation: BD Rhapsody System (BD Biosciences) Single-cell RNA sequencing: NEB Next UltraIIFS Library prep kit (New England Biolabs) and Illumina Novaseq 6000 sequencer Fluorescence and absorbance measurement: Nivo (PerkinElmer) Liquid chromatography-tandem mass spectrometry: Triple-quadrupole mass spectrometer (Shimadzu Corporation,LCMS-8060) Analysis of (oxidized) lipids: Orbitrap Exploris mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA) linked with Ultimate 3000 HPLC system (Thermo Fisher Scientific) Immunofluorescence staining analysis: NIH Image J software (version 1.57) Statistical analysis and visualization: R version 4.4.1 (2024-06-14) Statistical analysis: GraphPad Prism 10 (version 10.4.2) Visualization: Illustrator (version 29.0.1) Assessment of oxidized lipids: Qual Browser (Thermo Fisher Scientific) Metabolome analysis: Metaboanalyst 6.0 2 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
Immunofluorescence:Article Title: Senescence-associated lysosomal dysfunction impairs cystine deprivation-induced lipid peroxidation and ferroptosis
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Data analysis Akiko Takahashi Jun 27, 2025 Confocal microscopic data: ECLIPSE Ti2-E, Nikon AX Quantitative real-time PCR data: StepOnePlus PCR system (Applied Biosystems) Immunoblotting data: FUSION imaging system (Vilber-Lourmat) Histological data: Fluorescence microscope (Olymplus, BX51) Fluorescence imaging analysis: Operetta CLS (PerkinElmer) Flow cytometry: Spectral analyzer SA3800 (SONY) scRNA-Seq library preparation: BD Rhapsody System (BD Biosciences) Single-cell RNA sequencing: NEB Next UltraIIFS Library prep kit (New England Biolabs) and Illumina Novaseq 6000 sequencer Fluorescence and absorbance measurement: Nivo (PerkinElmer) Liquid chromatography-tandem mass spectrometry: Triple-quadrupole mass spectrometer (Shimadzu Corporation,LCMS-8060) Analysis of (oxidized) lipids: Orbitrap Exploris mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA) linked with Ultimate 3000 HPLC system (Thermo Fisher Scientific) Immunofluorescence staining analysis: NIH Image J software (version 1.57) Statistical analysis and visualization: R version 4.4.1 (2024-06-14) Statistical analysis: GraphPad Prism 10 (version 10.4.2) Visualization: Illustrator (version 29.0.1) Assessment of oxidized lipids: Qual Browser (Thermo Fisher Scientific) Metabolome analysis: Metaboanalyst 6.0 2 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
Staining:Article Title: Senescence-associated lysosomal dysfunction impairs cystine deprivation-induced lipid peroxidation and ferroptosis
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Data analysis Akiko Takahashi Jun 27, 2025 Confocal microscopic data: ECLIPSE Ti2-E, Nikon AX Quantitative real-time PCR data: StepOnePlus PCR system (Applied Biosystems) Immunoblotting data: FUSION imaging system (Vilber-Lourmat) Histological data: Fluorescence microscope (Olymplus, BX51) Fluorescence imaging analysis: Operetta CLS (PerkinElmer) Flow cytometry: Spectral analyzer SA3800 (SONY) scRNA-Seq library preparation: BD Rhapsody System (BD Biosciences) Single-cell RNA sequencing: NEB Next UltraIIFS Library prep kit (New England Biolabs) and Illumina Novaseq 6000 sequencer Fluorescence and absorbance measurement: Nivo (PerkinElmer) Liquid chromatography-tandem mass spectrometry: Triple-quadrupole mass spectrometer (Shimadzu Corporation,LCMS-8060) Analysis of (oxidized) lipids: Orbitrap Exploris mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA) linked with Ultimate 3000 HPLC system (Thermo Fisher Scientific) Immunofluorescence staining analysis: NIH Image J software (version 1.57) Statistical analysis and visualization: R version 4.4.1 (2024-06-14) Statistical analysis: GraphPad Prism 10 (version 10.4.2) Visualization: Illustrator (version 29.0.1) Assessment of oxidized lipids: Qual Browser (Thermo Fisher Scientific) Metabolome analysis: Metaboanalyst 6.0 2 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
|
Nikon is a verified supplier 